Q-Plex™ Human T Cell Cytokine HS (9-Plex)
Q-Plex™ Human T Cell Cytokine HS (9-Plex)
For measurement of IFNγ, IL-2, IL-4, IL-5, IL-10, IL-13, IL-17A, IL-21, TNFα
- Species Specificity: Human
- Validated Sample Type: Human Serum, EDTA/Heparin Plasma
- Sample Volume Required: Min. 25 µL
- Estimated Assay Length: 4.5 hours
- Intra-Assay Cross-Reactivity: No significant interference observed. <1% cross-reactivity observed.
- Assay Type: Sandwich
- Detection Method: Chemiluminescent
- Multiplex Format: 96-well solid plate
- Catalog #: 111933HU
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| IFNγ | Sandwich | 99 – 0.14 (pg/mL) | 69.3 (pg/mL) | 0.18 (pg/mL) | 0.10 (pg/mL) | 10% | 3% | 99% |
| IL-2 | Sandwich | 64 – 0.09 (pg/mL) | 44.8 (pg/mL) | 0.11 (pg/mL) | 0.09 (pg/mL) | 8% | 6% | 101% |
| IL-4 | Sandwich | 21 – 0.03 (pg/mL) | 14.7 (pg/mL) | 0.04 (pg/mL) | 0.02 (pg/mL) | 11% | 4% | 97% |
| IL-5 | Sandwich | 333 – 0.46 (pg/mL) | 233 (pg/mL) | 0.59 (pg/mL) | 0.46 (pg/mL) | 10% | 7% | 102% |
| IL-10 | Sandwich | 2680 – 3.68 (pg/mL) | 4.78 (pg/mL) | 2.39 (pg/mL) | 2.98 (pg/mL) | 11% | 3% | 99% |
| IL-13 | Sandwich | 333 – 0.46 (pg/mL) | 233 (pg/mL) | 0.59 (pg/mL) | 0.23 (pg/mL) | 11% | 5% | 105% |
| IL-17A | Sandwich | 5589 – 7.67 (pg/mL) | 3912 (pg/mL) | 9.97 (pg/mL) | 6.21 (pg/mL) | 9% | 5% | 104% |
| IL-21 | Sandwich | 250 - 0.34 (pg/mL) | 175 (pg/mL) | 1 (pg/mL) | 0.17 (pg/mL) | 12% | 9% | 95% |
| TNFα | Sandwich | 661 - 0.91 (pg/mL) | 462 (pg/mL) | 1.18 (pg/mL) | 0.46 (pg/mL) | 12% | 6% | 100% |
Product Overview
The Q-Plex™ Human T Cell Cytokine HS (9-Plex) is a fully quantitative plate-based multiplex ELISA for the detection of cytokines associated with T cell-mediated immunity.
T cells are one of the major lymphocyte arms of the adaptive immune system. Different subsets of T cells exist including Th1, Th2, Th17, and Tregs. Each subset of T cell is characterized by signature cytokine profiles as well as transcription factors and are specialized for protecting the host against different threats. T cell biology is studied in a variety of conditions including cancer, autoimmunity, allergy, and infectious diseases.
Product Documentation
Kit Content
Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
Reagents Include:
- Detection Mix
- Substrate
- Sample Diluents
- Streptavidin HRP
- Wash Buffer
Processing
All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.
Q-Plex™ arrays are developed and optimized to work with Q-View™ imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.
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