Q-Plex™ Human T Cell Cytokine HS (9-Plex)

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Q-Plex™ Human T Cell Cytokine HS (9-Plex)

For measurement of IFNγ, IL-2, IL-4, IL-5, IL-10, IL-13, IL-17, IL-21, TNFα

  • Species Specificity: Human
  • Validated Sample Type: Human Serum, EDTA/Heparin Plasma
  • Sample Volume Required: 4.5 hours
  • Estimated Assay Length: Min. 25 µL
  • Intra-Assay Cross-Reactivity:
  • Assay Type: Sandwich
  • Detection Method: Chemiluminescent
  • Multiplex Format: 96-well solid plate
  • Catalog #: 111933HU
Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Precision* (Inter-assay) Precision* (Intra-assay) Average* Linearity
IFNγSandwich 99 – 0.14 (pg/mL) 69.3 (pg/mL) 0.18 (pg/mL) 0.10 (pg/mL) 10% 3% 99%
IL-2Sandwich 64 – 0.09 (pg/mL) 44.8 (pg/mL) 0.11 (pg/mL) 0.09 (pg/mL) 8% 6% 101%
IL-4Sandwich 21 – 0.03 (pg/mL) 14.7 (pg/mL) 0.04 (pg/mL) 0.02 (pg/mL) 11% 4% 97%
IL-5Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.46 (pg/mL) 10% 7% 102%
IL-10Sandwich 2680 – 3.68 (pg/mL) 4.78 (pg/mL) 2.39 (pg/mL) 2.98 (pg/mL) 11% 3% 99%
IL-13Sandwich 333 – 0.46 (pg/mL) 233 (pg/mL) 0.59 (pg/mL) 0.23 (pg/mL) 11% 5% 105%
IL-17Sandwich 5589 – 7.67 (pg/mL) 3912 (pg/mL) 9.97 (pg/mL) 6.21 (pg/mL) 9% 5% 104%
IL-21Sandwich 250 - 0.34 (pg/mL) 175 (pg/mL) 1 (pg/mL) 0.17 (pg/mL) 12% 9% 95%
TNFαSandwich 661 - 0.91 (pg/mL) 462 (pg/mL) 1.18 (pg/mL) 0.46 (pg/mL) 12% 6% 100%

Product Overview

The Q-Plex™ Human T Cell Cytokine HS (9-Plex) is a fully quantitative plate-based multiplex ELISA for the detection of cytokines associated with T cell-mediated immunity.

T cells are one of the major lymphocyte arms of the adaptive immune system. Different subsets of T cells exist including Th1, Th2, Th17, and Tregs. Each subset of T cell is characterized by signature cytokine profiles as well as transcription factors and are specialized for protecting the host against different threats. T cell biology is studied in a variety of conditions including cancer, autoimmunity, allergy, and infectious diseases.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents Include:

  • Detection Mix
  • Substrate
  • Sample Diluents
  • Streptavidin HRP
  • Wash Buffer


All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.

Q-Plex™ arrays are developed and optimized to work with Q-View™ imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.

Other Equipment Recommendations

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Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

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Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.