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Human IL-2 Analyte Overview

Human IL-2

Also known as Interleukin 2

Interleukin-2 (IL-2) cytokine signaling molecule in the immune system that mediates the activity of white blood cells, particularly lymphocytes, during an immune response. IL-2 promotes the differentiation of T cells into memory T cells after exposure to a novel pathogen, making it a critical component of the active immune system and long-term immunity to disease. In the thymus, IL-2 supports the formation of regulatory T cells, which work to prevent autoimmune diseases by eliminating overactive immune cells. Since IL-2 helps increase immune cell production and regulate large scale activities of the immune system, it is commonly used as a cancer treatment for patients with certain kinds of cancers, including kidney and skin cancers.

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 5,213 – 7.15 pg/mL
Lower Limit of Detection (LLD): 4.12 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 465749HU

Features & Requirements

  • Measure IL-2 in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 5%

Sample Concentration (pg/ml) %CV
Sample 1 510.82 4%
Sample 2 35.56 4%
Sample 3 12.15 7%

 

Inter-assay (n=15)
Average Inter-assay CV: 6%

Sample Concentration (pg/ml) %CV
Sample 1 1916.94 6%
Sample 2 139.65 10%
Sample 3 488.43 3%

 

Dilutional Linearity

Average percent linearity: 99%
Percent linearity range: 82 – 110%

Dilution Factor % Recovery
Serum HA Serum HB Serum HC Pooled Plasma
2 87% 93% 82% 93%
4 108% 101% 103% 107%
8 107% 110% 98% 97%
Human il-2 linearity

Not Finding What You Need?

Customize a Kit

Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

Send in Your Samples

Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

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Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

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