Q-Plex™ SARS-CoV-2 Human IgG (5-Plex)
Q-Plex SARS-CoV-2 Human IgG (5-Plex)
For measurement of SARS-CoV-2 S1, SARS-CoV-2 S2, Sheep Fc (negative control), Anti-Human IgG (positive control), Nucleocapsid protein
- Species Specificity: Human
- Validated Sample Type: Human Serum, Plasma, and Dried Whole Blood Samples
- Sample Volume Required per Well: 2 µL
- Estimated Assay Length: 2 hours
- Specificity: See assay validation below
- Assay Type: Indirect
- Detection Method: Chemiluminescent
- Multiplex Format: 96-well plate
- Catalog #: 721649HU
| Analyte | Assay Type | Range | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|
| S1 | Indirect | 1000 - 1.3 U/mL | 1.3 U/mL | 14% | 6.2% | 105% |
| S2 | Indirect | 1000 - 1.3 U/mL | 1.3 U/mL | 14% | 13.2% | 101% |
| NP | Indirect | 1000 - 1.3 U/mL | 0.9 U/mL | 13% | 9% | 103% |
| SARS-CoV-2 Spike Proteins S1 and S2 IgG Assay vs. Molecular COVID-19 Test | ||
|---|---|---|
| N=360 | Confirmed Positive | Confirmed Negative |
| IgG Test Positive | 33 | 0 |
| IgG Test Negative | 1 | 326 |
| SARS-CoV-2 Nucleocapsid IgG Assay vs. Molecular COVID-19 Test | ||
|---|---|---|
| N=360 | Confirmed Positive | Confirmed Negative |
| IgG Test Positive | 32 | 19 |
| IgG Test Negative | 2 | 307 |
| S1 and S2 vs. PCR | S1 and S2 vs. PCR | |
|---|---|---|
| Sensitivity (PPA) | 97.1% | 94.1% |
| Specificity (NPA) | 100% | 94.2% |
| Positive Predictive Value (PPV) | 100% | 62.7% |
| Negative Predictive Value (NPV) | 99.7% | 99.4% |
Principle of the Assay
Why the nucleocapsid addition?
Product Information
Kit Content
Reagents Include:
- Detection Mix
- Calibrators (High, Low, Negative)
- Substrate
- Sample Diluents
- Streptavidin HRP
- Wash Buffer
Processing
Q-Plex™ Arrays are developed and optimized to work with Q-View™ Imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex Arrays.
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Our Response to COVID-19
As new variants of SARS-CoV-2 arise, the global response must change and adapt. Tracking the infection and our immune response towards it becomes increasingly important. Learn more about our response to COVID-19.
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