Human IL-4 Analyte Overview

Human IL-4

Also known as Interleukin 4, B cell-stimulatory factor-1

Interleukin 4 (IL-4) is a cytokine that has pleiotropic effects during active immune responses. Its primary role is to induce differentiation of helper T-cells and active B cells. IL-4’s other roles include regulating immunity, upregulating MHC class II production, supporting the proliferation of epithelial cells, and downregulating the production of Th1 cells, macrophages, and some lines of dendritic cells.  IL-4 activates two different receptor complexes, one expressed on hematopoietic cells and another found on nonhematopoietic cells. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, eosinophils, and basophils. Its responsibilities include cell proliferation, immunoglobulin class switch (IgG4, IgE of human B cells), survival, priming and chemotaxis of mast cells, eosinophils, and basophils. Dysregulation of IL-4 is implicated in many autoimmune disorders, particularly the development of allergies. Overexpression of the cytokine is associated with tumor growth in many kinds of cancer. People infected with HIV often produce higher levels of IL-4. Human, mouse, and rat IL-4 are species-specific in their activities.

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 1,676 – 2.3 pg/mL
Lower Limit of Detection (LLD): 1.79 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 466249HU

Features & Requirements

  • Measure IL-4 in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics


Intra-assay (n=20)
Average Intra-assay CV: 4%

Sample Concentration (pg/ml) %CV
Sample 1


Sample 2 27.57 4%
Sample 3 9.16 4%


Inter-assay (n=15)
Average Inter-assay CV: 6%

Sample Concentration (pg/ml) %CV
Sample 1 55.95 8%
Sample 2 649.82 4%
Sample 3 286.68 5%


Dilutional Linearity

Average percent linearity: 94%
Percent linearity range: 87 – 102%

Dilution Factor % Recovery
Serum HA Serum HB Serum HC Pooled Plasma
2 90% 87% 101% 87%
4 102% 96% 99% 98%
8 93% 91% 97% 92%
human il-4 linearity

Not Finding What You Need?

Customize a Kit

Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

Send in Your Samples

Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

Contact a Representative

Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.