Q-Plex™ Human Type 2 Immunity (5-Plex)
Q-Plex™ Human Type 2 Immunity (5-Plex)
For measurement of IL-4, IL-5, IL-10, IL-13, IL-33
- Species Specificity: Human
- Validated Sample Type: Human Serum, EDTA/Heparin Plasma
- Sample Volume Required: Min. 25 µL
- Estimated Assay Length: 2.25 hours
- Intra-Assay Cross-Reactivity: No significant interference observed. <1% cross-reactivity observed.
- Assay Type: Sandwich
- Detection Method: Chemiluminescent
- Multiplex Format: 96-well solid plate
- Catalog #: 114233HU
|Analyte||Assay Type||Calibrator Range||Upper Limit of Quantification (ULOQ)||Lower Limit of Quantification (LLOQ)||Limit of Detection||Precision* (Inter-assay)||Percision* (Intra-assay)||Average* Linearity|
|IL-4||Sandwich||1676 – 2.30 (pg/mL)||1173 (pg/mL)||3.0 (pg/mL)||1.79 (pg/mL)||6%||4%||94%|
|IL-5||Sandwich||3000 – 4.12 (pg/mL)||2100 (pg/mL)||5.3 (pg/mL)||2.90 (pg/mL)||10%||5%||95%|
|IL-10||Sandwich||8039 – 11.03 (pg/mL)||5627 (pg/mL)||14.3 (pg/mL)||10.99 (pg/mL)||7%||4%||103%|
|IL-13||Sandwich||3000 – 4.12 (pg/mL)||2100 (pg/mL)||5.3 (pg/mL)||4.12 (pg/mL)||6%||5%||98%|
|IL-33||Sandwich||350 - 0.48 (pg/mL)||245 (pg/mL)||0.62 (pg/mL)||0.24 (pg/mL)||15%||6%||92%|
The Q-Plex™ Human Type 2 Immunity (5-Plex) is a fully quantitative plate-based multiplex ELISA for the detection of cytokines associated with type 2 immunity.
Functions of type 2 immunity include protecting the host, maintaining metabolic homeostasis, suppressing type 1 inflammation, barrier defense, and tissue regeneration. Like other immune responses, excessive or chronic activation of type 2 immunity can be harmful to the host. Allergic disease, inability to defend against pathogens, and fibrosis have been described as harmful actions of type 2 immunity.
Producers of type 2 cytokines include macrophages, mast cells, T cells, macrophages, monocytes, basophils, eosinophils, and natural killer cells. A variety of immune and non-immune cells have been noted to produce type 2 cytokine responses in response epithelial damage, release of alarmins, and antigen presentation/recognition.
Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
- Detection Mix
- Sample Diluents
- Streptavidin HRP
- Wash Buffer
All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.
Q-Plex™ arrays are developed and optimized to work with Q-View™ imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.
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