Q-Plex™ Human Type 2 Immunity (5-Plex)

Q-Plex™ Human Type 2 Immunity (5-Plex)

For measurement of IL-4, IL-5, IL-10, IL-13, IL-33

  • Species Specificity: Human
  • Validated Sample Type: Human Serum, EDTA/Heparin Plasma
  • Sample Volume Required: Min. 25 µL
  • Estimated Assay Length: 2.25 hours
  • Intra-Assay Cross-Reactivity: No significant interference observed. <1% cross-reactivity observed.
  • Assay Type: Sandwich
  • Detection Method: Chemiluminescent
  • Multiplex Format: 96-well solid plate
  • Catalog #: 114233HU
Analyte Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Precision (Inter-assay) Percision (Intra-assay) Average Linearity
IL-4 Sandwich 1676 – 2.30 (pg/mL) 1173 (pg/mL) 3.0 (pg/mL) 1.79 (pg/mL) 6% 4% 94%
IL-5 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 2.90 (pg/mL) 10% 5% 95%
IL-10 Sandwich 8039 – 11.03 (pg/mL) 5627 (pg/mL) 14.3 (pg/mL) 10.99 (pg/mL) 7% 4% 103%
IL-13 Sandwich 3000 – 4.12 (pg/mL) 2100 (pg/mL) 5.3 (pg/mL) 4.12 (pg/mL) 6% 5% 98%
IL-33 Sandwich 350 - 0.48 (pg/mL) 245 (pg/mL) 0.62 (pg/mL) 0.24 (pg/mL) 15% 6% 92%

Product Overview

The Q-Plex™ Human Type 2 Immunity (5-Plex) is a fully quantitative plate-based multiplex ELISA for the detection of cytokines associated with type 2 immunity.

Functions of type 2 immunity include protecting the host, maintaining metabolic homeostasis, suppressing type 1 inflammation, barrier defense, and tissue regeneration. Like other immune responses, excessive or chronic activation of type 2 immunity can be harmful to the host. Allergic disease, inability to defend against pathogens, and fibrosis have been described as harmful actions of type 2 immunity.

Producers of type 2 cytokines include macrophages, mast cells, T cells, macrophages, monocytes, basophils, eosinophils, and natural killer cells. A variety of immune and non-immune cells have been noted to produce type 2 cytokine responses in response epithelial damage, release of alarmins, and antigen presentation/recognition.

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents Include:

  • Detection Mix
  • Substrates
  • Sample Diluents
  • Streptavidin HRP
  • Wash Buffer


All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.

Q-Plex™ arrays are developed and optimized to work with Q-View™ imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.

Other Equipment Recommendations

Publication Library

Search our publication library for all the peer-reviewed journals and research products that have utilized Q-Plex™ technology here.

In-Kit Certificate of Analysis

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Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

Send in Your Samples

Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

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Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

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