Human IL-10 Analyte Overview
Human IL-10
Also known as Interleukin 10, human cytokine synthesis inhibitory factor (CSIF), CSIF, IL10A, MGC126450, MGC126451, TGIF
Interleukin 10 (IL-10) is a class-2 anti-inflammatory cytokine, also known as human cytokine synthesis inhibitory factor (CSIF). It impacts many areas of immunoregulation and inflammation, with numerous pleiotropic effects in the immune system. As an anti-inflammatory, it downregulates MHC class II expression, inhibits the synthesis and activity of many inflammatory cytokines, and reduces co-stimulatory factors on macrophages. It also enhances the release of other anti-inflammatory mediators by monocytes and macrophage, particularly during exercise. It enhances B cell proliferation, differentiation, survival, and antibody production. IL-10 has anti-tumor effects, reducing tumor growth and decreasing metastatic burden in the body. IL-10 deficiency is associated with chronic inflammatory and autoimmune diseases, including multiple sclerosis, Crohn’s disease, inflammatory bowel disease, psoriasis, and rheumatoid arthritis. IL-10 is currently under investigation as a potential therapeutic for many conditions, including cancer treatments and reducing symptoms in inflammatory disorders.
Q-Plex™ Details
Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 8,039 – 11.09 pg/mL
Lower Limit of Detection (LLD): 10.99 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 464749HU
Features & Requirements
- Measure IL-10 in 2.25 hours
- Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
- Requires the Q-View™ Imaging System for analysis
Assay Performance Characteristics
Precision
Intra-assay (n=20)
Average Intra-assay CV: 4%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 |
424.12 |
2% |
| Sample 2 | 31.08 | 3% |
| Sample 3 | 10.08 | 6% |
Inter-assay (n=15)
Average Inter-assay CV: 7%
| Sample | Concentration (pg/ml) | %CV |
| Sample 1 | 46.66 | 6% |
| Sample 2 | 33.06 | 7% |
| Sample 3 | 13.44 | 8% |
Dilutional Linearity
Average percent linearity: 103%
Percent linearity range: 93 – 112%
| Dilution Factor | % Recovery | |||
| Serum HA | Serum HB | Serum HC | Pooled Plasma | |
| 2 | 103% | 100% | 106% | 112% |
| 4 | 105% | 104% | 105% | 108% |
| 8 | 100% | 99% | 93% | 99% |
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