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Human IL-17A Analyte Overview

Human IL-17A

Also known as Interleukin 17, IL-17a

Interleukin 17 (IL-17) are a small family of similarly structured cytokines that plays a role in pro-inflammatory responses of the immune system, particularly allergic reactions. Each variant of IL-17 appears to have slightly nuanced function in different organs, but on the whole the cytokines behave similarly. IL-17 induces inflammation by stimulating the secretion of pro-inflammatory molecules such as IL-6, IL-8, GM-CSF, IL-1, TNF-α, and MCP-1 from their respective cell types. For this reason, IL-17 is linked to inflammatory and autoimmune diseases such as rheumatoid arthritis, asthma, lupus, psoriasis, and multiple scelrosis. Overactivity of IL-17 is commonly found in severe cases of asthma and psoriasis, where it contributes to inflammation that damages the airway and skin, respectively. IL-17 suppression is under investigation as a clinical target for reducing symptoms of inflammatory diseases, improving recovery following stroke, and treatment of some types of cancer.

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 17,722 – 22.92 pg/mL
Lower Limit of Detection (LLD): 22.57 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 465349HU

Features & Requirements

  • Measure IL-17 in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 6%

Sample Concentration (pg/ml) %CV
Sample 1

930.54

5%
Sample 2 65.05 4%
Sample 3 19.47 8%

 

Inter-assay (n=15)
Average Inter-assay CV: 7%

Sample Concentration (pg/ml) %CV
Sample 1 148.77 9%
Sample 2 903.04 7%
Sample 3 993.78 6%

 

Dilutional Linearity

Average percent linearity: 95%
Percent linearity range: 74 – 114%

Dilution Factor % Recovery
Cell Culture Supernatant Serum HD Stripped Serum Pooled Plasma
2 112% 112% 94% 95%
4 97% 111% 100% 98%
8 91% 104% 90% 93%
human il-17 linearity

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