Human IL-8 Analyte Overview

Human IL-8

Also known as Interleuken-8, Chemokine Ligand 8, CXCL8

Interleukin 8 (IL-8), also known as CXCL8, is a chemokine that plays a role in the recruitment and regulation of white blood cells during an innate immune response. It is a strong chemoattractant for neutrophils and other granulocytes, bringing them to the site of infection and then stimulating them to engage in phagocytosis. It is commonly secreted by macrophages that have identified a foreign pathogen but may be emitted by any cell with toll-like receptors. IL-8 plays a key role in metastasis; it was first identified as a leukocyte chemoattractant that progresses melanoma by directly inducing angiogenesis and migration of melanoma cells. Serum IL-8 levels are directly correlated with the degree of metastatic melanoma and overall tumor count. High levels of IL-8 in a pregnant mother are associated with schizophrenia in her child and high levels in adults with schizophrenia reduces the effectiveness of antipsychotic medications. IL-8 dysregulation is also involved in cystic fibrosis, where it recruits an excessive number of white blood cells to the lungs, resulting in tissue damage.

IL-8 Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 2,000 – 2.74 pg/ml
Lower Limit of Detection (LLD): 2.70 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 466749HU

N

Measure IL-8 in 2.25 hours

N

Sensitive, reproducible, and convenient 96-well format requiring low sample volumes

N

Requires the Q-View™ Imaging System for analysis

IL-8 Assay Performance Characteristics

Precision

Intra-assay (n=20)
Average Intra-assay CV: 12%

Sample Concentration (pg/ml) %CV
Sample 1

360.08

11%
Sample 2 30.85 11%
Sample 3 9.47 14%

 

Inter-assay (n=15)
Average Inter-assay CV: 12%

Sample Concentration (pg/ml) %CV
Sample 1 137.1 7%
Sample 2 1937.14 11%
Sample 3 2034.33 11%

 

Dilutional Linearity

Average percent linearity: 104%
Percent linearity range: 94 – 111%

Dilution Factor % Recovery
Serum HA Serum HB Serum HC Pooled Plasma
2 105% 117% 99% 105%
4 107% 97% 102% 114%
8 100% 81% 88% 88%
Human il-8 linearity

Q-Plex Multiplexed Products featuring IL-8

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