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Human TNFα Analyte Overview

Human TNFα

Also known as TNF alpha, Tumor Necrosis Factor Alpha, Cachexin, Cachetin, TNFSF2

Tumor necrosis factor alpha (TNFα) is a prototypic ligand of the TNF superfamily. It is a versatile cytokine that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNFα is an important endogenous pyrogen, its release quickly induces fever. It also causes apoptotic cell death, cachexia, inflammation, and has the ability to inhibit tumorigenesis and viral replication. It is chemoattractive to many immune cells, particularly neutrophils. Cell surface TNFα can induce the lysis of neighboring tumor cells and virus infected cells and has the ability to generate its own downstream cell signaling. It is produced chiefly by activated macrophages, although it is produced in low levels by a wide variety of immune, epithelial, endothelial, and tumor cells such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFα also plays an important role in metabolism, with the ability to induce insulin resistance and regulate appetite through interactions with the hypothalamus. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer’s disease, inflammatory bowel disease, arthritis, cancer, and psoriasis.

Q-Plex™ Details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 5,954 – 8.17 pg/mL
Lower Limit of Detection (LLD): 7.74 pg/mL
Volume Required Per Well: Min. 25µL
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog #: 469749HU

Features & Requirements

  • Measure TNFα in 2.25 hours
  • Sensitive, reproducible, and convenient 96-well format requiring low sample volumes
  • Requires the Q-View™ Imaging System for analysis

Assay Performance Characteristics

Precision

Sample Concentration (pg/ml) %CV
Sample 1 632.70 6%
Sample 2 50.51 6%
Sample 3 14.14 7%

Intra-assay (n=20)
Average Intra-assay CV: 6%

Sample Concentration (pg/ml) %CV
Sample 1 2320.06 7%
Sample 2 2607.76 5%
Sample 3 720.04 7%

Inter-assay (n=15)
Average Inter-assay CV: 6% 

Dilutional Linearity

Dilution Factor % Recovery
PI Plasma Serum HH Pooled Plasma Serum HC
2 95% 92% 93% 99%
4 101% 98% 97% 104%
8 96% 100% 97% 100%

Average percent linearity: 98%
Percent linearity range: 92 – 104%

human tnfa linearity

Not Finding What You Need?

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Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

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Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

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