Human TNFα Analyte Overview

Human TNFa

Also known as TNFa, TNF alpha, TNF a, TNFA, TNF-a, Tumor Necrosis Factor Alpha, Cachexin, Cachetin, TNFSF2

Tumor necrosis factor alpha (TNFα) is a prototypic ligand of the TNF superfamily. It is a versatile cytokine that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNFα is an important endogenous pyrogen, its release quickly induces fever. It also causes apoptotic cell death, cachexia, inflammation, and has the ability to inhibit tumorigenesis and viral replication. It is chemoattractive to many immune cells, particularly neutrophils. Cell surface TNFα can induce the lysis of neighboring tumor cells and virus infected cells and has the ability to generate its own downstream cell signaling. It is produced chiefly by activated macrophages, although it is produced in low levels by a wide variety of immune, epithelial, endothelial, and tumor cells such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFα also plays an important role in metabolism, with the ability to induce insulin resistance and regulate appetite through interactions with the hypothalamus. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer’s disease, inflammatory bowel disease, arthritis, cancer, and psoriasis.

TNFα Q-Plex™ details

Species: Human
Assay Type: Sandwich
Sample Type: Human Serum, EDTA Plasma
Assay Range: 5,954 – 8.17 pg/ml
Lower Limit of Detection (LLD): 7.74 pg/ml
Volume Required Per Well: Min 25µl
Detection Method: Chemiluminescent
Singleplex Format: 96-well plate
Catalog No: 469749HU


Measure TNFα in 2.25 hours


Sensitive, reproducible, and convenient 96-well format requiring low sample volumes


Requires the Q-View™ Imaging System for analysis

TNFα Assay Performance Characteristics


Intra-assay (n=20)
Average Intra-assay CV: 6%

Sample Concentration (pg/ml) %CV
Sample 1 632.70 6%
Sample 2 50.51 6%
Sample 3 14.14 7%


Inter-assay (n=15)
Average Inter-assay CV: 6%

Sample Concentration (pg/ml) %CV
Sample 1 2320.06 7%
Sample 2 2607.76 5%
Sample 3 720.04 7%


Dilutional Linearity

Average percent linearity: 98%
Percent linearity range: 92 – 104%

Dilution Factor % Recovery
PI Plasma Serum HH Pooled Plasma Serum HC
2 95% 92% 93% 99%
4 101% 98% 97% 104%
8 96% 100% 97% 100%
human tnfa linearity

Q-Plex Multiplexed Products featuring TNFα

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