Q-Plex™ Human Environmental Enteric Dysfunction (11-Plex)
Q-Plex Human Environmental Enteric Dysfunction (11-Plex)
For measurement of AGP, CD-14, CRP, Ferritin, FGF-21, HRP2, IFABP, IGF-1, RBP4, sTfR, Thyroglobulin
- Species Reactivity: Human
- Validated Sample Type: Serum, Heparin Plasma
- Sample Volume Required: 5 µL
- Estimated Assay Length: 3.5 hours
- Cross-Reactivity and Interference: No significant interference observed. <1% cross-reactivity observed.
- Assay Type: Varies (See Performance and Validation Data table below)
- Detection Method: Chemiluminescent
- Multiplex Format: 96-well solid plate
- Catalog #: 569849HU
| Analytes | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| AGP | Competitive | 0.36 - 0.0015 (g/L) | 0.42 (g/L) | 0.0006 (g/L) | 0.0019 (g/L) | 12% | 5% | 100-114%(108%) |
| CD-14 | Competitive | 1750 - 2.4 (ng/mL) | 1750 (ng/mL) | 2.40 (ng/mL) | 2.4 (ng/mL) | 11% | 4% | 85-104%(93%) |
| CRP | Competitive | 5.61 - 0.008 (mg/L) | 21 (mg/L) | 0.0288 (mg/L) | 0.1112 (mg/L) | 14% | 3% | 91-111%(99%) |
| Ferritin | Sandwich | 115 - 0.16 (μg/L) | 99 (µg/L) | 0.14 (µg/L) | 0.2057 (µg/L) | 12% | 4% | 85-110% (103%) |
| FGF-21 | Sandwich | 800 - 0.11 (pg/mL) | 1800 (pg/mL) | 2.47 (pg/mL) | 1.1 (pg/mL) | 12% | 4% | 96-104%(101%) |
| HRP2 | Sandwich | 0.955 - 0.0013 (μg/L) | 0.85 (µg/L) | 0.0012 (µg/L) | 0.0017 (µg/L) | 13% | 6% | 83-117%(106%) |
| IFABP | Sandwich | 3000 - 4.1 (pg/mL) | 2800 (pg/mL) | 3.84 (pg/mL) | 4.12 (pg/mL) | 10% | 6% | 87-108%(97%) |
| IGF-1 | Sandwich | 198 - 0.27 (ng/mL) | 186 (ng/mL) | 0.26 (ng/mL) | 0.27 (ng/mL) | 13% | 7% | 100-131%(117%) |
| RBP4 | Competitive | 1.4 - 0.006 (μmol/L) | 1.9 (μmol/L) | 0.0026 (μmol/L) | 0.0054 (μmol/L) | 11% | 6% | 81-101%(94%) |
| sTfR | Sandwich | 107.525 - 0.15 (mg/L) | 104 (mg/L) | 0.14 (mg/L) | 0.1917 (mg/L) | 15% | 6% | 95-111%(101%) |
| Thyroglobulin | Sandwich | 13.7 - 0.019 (μg/L) | 12 (µg/L) | 0.02 (µg/L) | 0.0244 (µg/L) | 13% | 4% | 95-110%(103%) |
Product Overview
Environmental Enteric Dysfunction (EED) is characterized by inflammation at mucosal sites, reduced intestinal barrier integrity, and poor absorption of nutrients. EED is common among people living in low-resource settings with high enteric-pathogen prevalence and poor sanitation. Complications related to EED include stunted growth, reduced responsiveness to vaccines, and impaired cognitive development. Children are therefore especially at risk as these severe complications increase susceptibility to other childhood diseases such as pneumonia, acute diarrhea, and malaria.
Developed in collaboration with PATH, the Q-Plex Human Environmental Enteric Dysfunction (11-Plex) was designed to be a tool to help with identifying people with EED at high risk for additional complications. The array provides researchers an easy-to-use and cost-effective means of generating a profile of nutritional and inflammatory biomarkers for each sample.
| Indications | Marker | Function |
|---|---|---|
| Growth and Vaccine Response | Intestinal fatty acid binding protein (I-FABP) | Small intestine damage |
| Soluble CD14 (sCD14) | White blood cell activation | |
| Insulin-like growth factor 1 (IGF-1) | Bone and tissue growth | |
| Fibroblast growth factor 21 (FGF21) | Modulates metabolic pathways | |
| Systemic Inflammation | Alpha-1-acid glycoprotein (AGP) | Inflammation |
| C-reactive protein (CRP) | Inflammation | |
| Nutrition | Ferritin | Iron stores |
| Soluble transferrin receptor (sTfR) | Iron deficiency | |
| Retinol binding protein 4 (RBP4) | Vitamin A transport | |
| Thyroglobulin (Tg) | Iodine nutritional status | |
| Malaria | Histidine-rich protein 2 (HRP2) | Presence/absence of malaria |
Product Documentation
Kit Content
Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.
Reagents Include:
- Calibrator
- Competitor
- Detection Mix
- Substrates
- Sample Diluents
- Streptavidin HRP
- Wash Buffer
Processing
All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.
Q-Plex™ arrays are developed and optimized to work with Q-View™ imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.
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