Q-Plex™ Human Environmental Enteric Dysfunction (11-Plex)

Q-Plex Human Environmental Enteric Dysfunction (11-Plex)

For measurement of AGP, CD-14, CRP, Ferritin, FGF-21, HRP2, IFABP, IGF-1, RBP4, sTfR, Thyroglobulin 

  • Species Reactivity: Human
  • Validated Sample Type: Serum, Heparin Plasma
  • Sample Volume Required: 5 µL
  • Estimated Assay Length: 3.5 hours
  • Cross-Reactivity and Interference: No significant interference observed. <1% cross-reactivity observed. 
  • Assay Type: Varies (See Performance and Validation Data table below)
  • Detection Method: Chemiluminescent
  • Multiplex Format: 96-well solid plate
  • Catalog #: 569849HU
Analytes Assay Type Calibrator Range Upper Limit of Quantification (ULOQ) Lower Limit of Quantification (LLOQ) Limit of Detection Precision (Inter-assay) Precision (Intra-assay) Average Linearity
AGP Competitive 0.36 - 0.0015 (g/L) 0.42 (g/L) 0.0006 (g/L) 0.0019 (g/L) 12% 5% 100-114%(108%)
CD-14 Competitive 1750 - 2.4 (ng/mL) 1750 (ng/mL) 2.40 (ng/mL) 2.4 (ng/mL) 11% 4% 85-104%(93%)
CRP Competitive 5.61 - 0.008 (mg/L) 21 (mg/L) 0.0288 (mg/L) 0.1112 (mg/L) 14% 3% 91-111%(99%)
Ferritin Sandwich 115 - 0.16 (μg/L) 99 (µg/L) 0.14 (µg/L) 0.2057 (µg/L) 12% 4% 85-110% (103%)
FGF-21 Sandwich 800 - 0.11 (pg/mL) 1800 (pg/mL) 2.47 (pg/mL) 1.1 (pg/mL) 12% 4% 96-104%(101%)
HRP2 Sandwich 0.955 - 0.0013 (μg/L) 0.85 (µg/L) 0.0012 (µg/L) 0.0017 (µg/L) 13% 6% 83-117%(106%)
IFABP Sandwich 3000 - 4.1 (pg/mL) 2800 (pg/mL) 3.84 (pg/mL) 4.12 (pg/mL) 10% 6% 87-108%(97%)
IGF-1 Sandwich 198 - 0.27 (ng/mL) 186 (ng/mL) 0.26 (ng/mL) 0.27 (ng/mL) 13% 7% 100-131%(117%)
RBP4 Competitive 1.4 - 0.006 (μmol/L) 1.9 (μmol/L) 0.0026 (μmol/L) 0.0054 (μmol/L) 11% 6% 81-101%(94%)
sTfR Sandwich 107.525 - 0.15 (mg/L) 104 (mg/L) 0.14 (mg/L) 0.1917 (mg/L) 15% 6% 95-111%(101%)
Thyroglobulin Sandwich 13.7 - 0.019 (μg/L) 12 (µg/L) 0.02 (µg/L) 0.0244 (µg/L) 13% 4% 95-110%(103%)

Product Overview

Environmental Enteric Dysfunction (EED) is characterized by inflammation at mucosal sites, reduced intestinal barrier integrity, and poor absorption of nutrients. EED is common among people living in low-resource settings with high enteric-pathogen prevalence and poor sanitation. Complications related to EED include stunted growth, reduced responsiveness to vaccines, and impaired cognitive development. Children are therefore especially at risk as these severe complications increase susceptibility to other childhood diseases such as pneumonia, acute diarrhea, and malaria.

Developed in collaboration with PATH, the Q-Plex Human Environmental Enteric Dysfunction (11-Plex) was designed to be a tool to help with identifying people with EED at high risk for additional complications. The array provides researchers an easy-to-use and cost-effective means of generating a profile of nutritional and inflammatory biomarkers for each sample.

Path Logo
Indications Marker Function
Growth and Vaccine Response Intestinal fatty acid binding protein (I-FABP) Small intestine damage
Soluble CD14 (sCD14) White blood cell activation
Insulin-like growth factor 1 (IGF-1) Bone and tissue growth
Fibroblast growth factor 21 (FGF21) Modulates metabolic pathways
Systemic Inflammation Alpha-1-acid glycoprotein (AGP) Inflammation
C-reactive protein (CRP) Inflammation
Nutrition Ferritin Iron stores
Soluble transferrin receptor (sTfR) Iron deficiency
Retinol binding protein 4 (RBP4) Vitamin A transport
Thyroglobulin (Tg) Iodine nutritional status
Malaria Histidine-rich protein 2 (HRP2) Presence/absence of malaria

Kit Content

Each kit contains a 96-well plate, featuring the relevant biomarker panel in each well, and all reagents required to perform testing.

Reagents Include:

  • Calibrator
  • Competitor
  • Detection Mix
  • Substrates
  • Sample Diluents
  • Streptavidin HRP
  • Wash Buffer


All Quansys Q-Plex™ Multiplex and Singleplex assays require the use of the Q-View™ software to read and interpret the test results. A fully-functional, free trial version of the software is available to download, install, and use to analyze your first kit(s). At the end of the trial period, a purchased license is required to continue the use of the Q-View software.

Q-Plex™ arrays are developed and optimized to work with Q-View™ imagers. Quansys does not guarantee the results obtained from other imagers and not all imagers are compatible with Quansys Q-Plex arrays.

Other Equipment Recommendations

    Publication Library

    Search our publication library for all the peer-reviewed journals and research products that have utilized Q-Plex™ technology here.

    In-Kit Certificate of Analysis

    Search and download Certificates of Analysis for your Quansys Biosciences kits here.

    Order Your Kit Today!

    Not Finding What You Need?

    Customize a Kit

    Not seeing the biomarker or analyte you need? Customize your kit! The Q-Plex™ Custom Assays are fully quantitative ELISA-based Chemiluminescent assay kits allowing the concurrent measurement of up to 18 biomarkers or analytes.

    Send in Your Samples

    Choose up to 18 different biomarkers that will work best for your research. Send in your samples and let us run them for you. 

    Contact a Representative

    Have questions about the kit or interested in more information? Contact one of our representative to get all your questions answered. 

    As researchers and problem solvers ourselves, we understand the value of sound data. We are proud to be a part of research that can better the world. We value the relationships, partnerships, and friendships that we have built with the people who trust and use our technology. We are committed to building these relationships. You can count on us to answer the phone and take time to thoroughly address questions or concerns about any of our products. In an industry that is reputed for grandiose claims, we trust that our quality standards and our customer service set us apart from the competition. If you’re happy with our products or if you think we can do something better, we hope you will let us know.