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Getting the most from Q-View Software

Getting the most fro Q-View Software

New look and touch screen compatible, but still three easy steps in the software to obtain concentrations:

Image Processing

Acquire/import images and align a plate overlay.
Well Assignment: Assign wells a type, name (optional), and dilution factor.
Data Analysis: Customize and copy or export the automatically generated concentrations, statistics, and charts.
Let’s Practice
– You’ve just finished the last step of the assay:

Capture the image(s)

Acquire on a Q-View Imager or Import a grayscale 16-bit TIFF. New Image Processing produces lower %CVs at the low end of the curve and eliminates stacking.

Input the Software Product Code

Found on the kit product card.

Place the overlay

Use Image Options to optimize image gamma, zoom, and orientation for your convenience. Use the Overlay Options tools to align the plate overlay. Always finish with Auto-Adjust Spots. (Also Blot Tools)

Assign Wells

Use Sequential Naming and Templates to quickly assign repeat layouts. Sample Controls can be assigned if they are part of the product definition.

Analyze Data

Click Perform Analysis to fit using the new optimal regression settings or modify via dialog, default assay limits. Then choose Statistics & optimal dilution, mask outliers, compare curve fit and weighting options. Example use of new Help button. Copy or Export Charts or data out as needed.

Data Analysis Troubleshooting

If a curve or sample did not perform as expected, first ensure that all plate overlay spots are aligned, the image has white spots on a black background, and dilution factors have been assigned. Then check the actual data, not just visual cues in the image.

Evaluate the Standard Curve

Check the standard curve charts, statistics for outliers to mask. Also consider masking a standard curve point if: %CV > 20%, or %Backfit ≠ 80% – 120%.
Check for signs of compression: Are there saturated points? Are there repeating pixel intensities?

Evaluate Sample Signals

Are the assay limits set to the ULOQ & LLOQ and does the signal fall on the linear part of the curve?
Is there high well or Negative Control background? Toggle Negative Well Subtraction setting.
Did sample controls, if present, pass?
Re-optimize the Curve Fit if Needed: Compare various regression models, weighting, and masking options.

Previous Tips for Data Analysis in the Q-View™ Software