Performing Western and Dot Blots on the Q-View Imager
While the Q-View™ Imager was exclusively designed and developed to be used with Q-Plex™ Arrays, some customers have been successful in using the device for western and dot blot imaging. Due to the numerous methods and reagents available, we are not able to provide specific steps on how to do this.
See below for a few tips so if you do choose to use the Q-View Imager for this purpose. Please keep in mind, some optimization may be required.
There are numerous chemiluminescent substrates available on the market of a variety of quality and performance. The substrate used in the Q-Plex™ kits is very sensitive and stable. The customers that have had good success with the Q-View Imager have used this substrate. From what we have observed, some of the less sensitive substrates are not acceptable for this application. Please contact your sales representative to request additional substrate.
The camera used in the Q-View Imager can do long exposures. We recommend doing multiple exposures, some short and some longer. It may be necessary to do exposures up to five minutes or longer.
Streptavidin HRP (SHRP)
While every experiment is different, we recommend maximizing the concentration of SHRP to achieve the desired sensitivity.